Figure 12.

Post-mortem evaluation of GRPs’ graft localization. Immunohistochemical staining in the brain of C57BL/6 (allogeneic) mouse model after 14 days of observation in the group where PiF was applied: The GFP+ cells (green) were observed in the midbrain tissue. We used an anti-GFP antibody (secondary antibody pertaining to red fluorescence Alexa Fluor 594 nm, Abcam, Cambridge, UK) to assess if the signal is specific (A,B). Abbreviations: GFP-green fluorescent protein; GRP-glial-restricted progenitor cell; PiF-preimplantation factor.