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. 2021 Jun 29;11(7):631. doi: 10.3390/life11070631

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PAG + L-cysteine (PL) pretreatment preserves SERCA2 level after ischemia/reperfusion. (a) Western blot of SERCA2in heart lysates from the control, I/R and PAG + L-cysteine + I/R (PL + I/R) hearts. (Original western blot see supplementary Figure S1) (b) Densitometry of SERCA2 protein normalized to GAPDH. n = 6 to 8 in each group. The control hearts were perfused by Langendorff preparation about 20 min, I/R hearts underwent 20 min of global ischemia followed by 40 min of reperfusion. Then, lysates for WB were prepared. * p < 0.05, ** p < 0.01 as calculated by one-way ANOVA with post hoc Tukey HSD test.