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. 2021 Jun 29;11(7):631. doi: 10.3390/life11070631

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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GSH (a) and GSSG (b) levels in the heart homogenates. Rats were treated with propargyl glycine and L-cysteine (PL) or with D,L-buthionine-(S,R)-sulfoximine +PL (BSO + PL), and then isolated hearts were submitted to Langendorffpreparation and underwent 20 min of global ischemia followed by 40 min of reperfusion (I/R). Control hearts were perfused by Langendorff preparation about 20 min. Then, probes for glutathione measurement were prepared. n = 6 to 8 in each group. * p < 0.05, ** p < 0.01 as calculated by Kruskal-Wallis with Mann-Whitney post hoc analysis.