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. 2021 Jun 29;11(7):631. doi: 10.3390/life11070631

Figure 5.

Figure 5

PAG+ L-cysteine pretreatment inhibitedfatty acids β-oxidation in I/R hearts. (a) Western blot of pACC at Ser79, ACC, pAMPK at Thr172, total AMPK, PPARa, pHSL at Ser565 andtotal HSL in heart lysates from control, I/R and PAG + L-cysteine + I/R (PL + I/R) groups. (Original western blot see supplementary Figure S2) (b)Densitometry of total ACC normalized to GAPDH. (c) Densitometry of pACC at Ser79 normalized to total ACC. (d)Densitometry of total AMPK normalized to GAPDH. (e) Densitometry of pAMPK at Thr172 normalized to total AMPK. (f)Densitometry of pHSL at Ser565normalized to total HSL. (g)Densitometry of total PPARa normalized to GAPDH. The data are expressed as the mean ± SEM of arbitrary fold of change relative to control levels. n = 6 to 8 per group. The control hearts were perfused by Langendorff preparation about 20 min, I/R heart underwent 20 min of global ischemia followed by 40 min of reperfusion. Then, lysates for WB were prepared. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc Tukey HSD test).