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. 2021 Jun 29;11(7):631. doi: 10.3390/life11070631

Figure 6.

Figure 6

PAG+ L-cysteine pretreatment promoted glucose consumption and glycolysis in I/R hearts. (a) Western blot ofpAKT at Ser 473, total AKT, pAS160 atThr642, total AS160,GLUT4 and PDK1 in whole heart lysates from control, I/R and PAG + L-cysteine + I/R rats. (Original western blot see supplementary Figure S3) (b) Densitometry of total AKT normalized to GAPDH. (c) Densitometry of pAKT at Ser473 normalized to total AKT. (d) Densitometry of total AS160 normalized to GAPDH. (e) Densitometry of pAS160 at Thr642 normalized to total AS160. (f) Densitometry of GLUT4 normalized to GAPDH. (g) Densitometry of total PDK1 to GAPDH. The data are expressed as the mean ± SEM of arbitrary fold of change relative to control levels. n = 6 to 8 per group. Control hearts were perfused by Langendorff preparation about 20 min, I/R heart underwent 20 min of global ischemia followed by 40 min of reperfusion. Then, lysates for WB were prepared. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc Tukey HSD test).