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. 2021 Jun 29;11(7):631. doi: 10.3390/life11070631

Figure 8.

Figure 8

PAG + L-cysteine pretreatment does not affect mTOR, MAPK/Erk1/2 and canonical Wnt signaling activity in the I/R hearts. (a) Western blot of pmTOR at Ser 2448, total mTOR, pErk1/2, total Erk1/2, pPKA, total PKA, active β-catenin, total β-catenin, pGSK3β at Ser21/9 and total GSK3β in whole heart lysates from the control, I/R and PAG + L-cysteine + I/R rats. (Original western blot see supplementary Figure S5) (b) Densitometry of total mTOR normalized to GAPDH. (c) Densitometry of pmTOR at Ser2448 normalized to mTOR. (d) Densitometry of total Erk1/2 normalized to GAPDH. (e) Densitometry of pErk1/2 normalized to total Erk1/2. (f) Densitometry of total PKA normalized to GAPDH. (g) Densitometry of pPKA normalized to total PKA. (h) Densitometry of activeβ-catenin normalized to total β-catenin. (i) Densitometry of total β-catenin normalized to GAPDH. (j) Densitometry of total GSK3α/β normalized to GAPDH. (k) Densitometry of pGSK3α/βatSer21/9 normalized to total GSK3α/β. The data are expressed as the mean ± SEM of arbitrary fold of change relative to control levels. n = 6 to 8 per group. Control hearts were perfused by Langendorff preparation about 20 min, I/R heart underwent 20 min of global ischemia followed by 40 min of reperfusion. Then, lysates for WB were prepared. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc Tukey HSD test).