Table 1.
Biochemical indexes of rat cardiac tissue.
| Index | Control (n = 8) |
I/R (n = 10) |
PAG + L-cysteine (n = 5) |
PAG + L-cysteine + I/R (n = 7) |
|---|---|---|---|---|
| O2−, nmol mg−1 min−1 |
2.63 ± 0.08 | 8.72 ± 0.57 *** | 1.61 ± 0.05 *** | 2.37 ± 0.11 ### |
| H2O2, pmol mg−1 |
0.79 ± 0.04 | 2.27 ± 0.26 *** | 0.69 ± 0.02 | 1.15 ± 0.11 ## |
| Diene conjugates, ng mg−1 |
3.59 ± 0.25 | 8.84 ± 0.48 *** | 0.92 ± 0.12 *** | 5.24 ± 0.63 ### |
| cNOS activity, pmol mg−1 min−1 |
7.51 ± 0.19 | 1.36 ± 0.09 *** | 8.57 ± 0.30 * | 2.38 ± 0.34 ## |
| iNOS activity, pmol mg−1 min−1 |
2.64 ± 0.15 | 7.07 ± 0.20 *** | 1.68 ± 0.04 *** | 3.86 ± 0.26 ### |
| NO2−, pmol mg−1 |
361.8 ± 17.9 | 131.5 ± 12.2 *** | 440.1 ± 5.2 ** | 343.4 ± 5.17 ### |
| NO3−, nmol mg−1 |
10.92 ± 0.21 | 22.27 ± 0.88 *** | 5.24 ± 0.13 *** | 9.95 ± 0.40 ### |
| H2S, pmol mg−1 |
17.56 ± 1.26 | 96.80 ± 7.90 *** | 14.56 ± 0.77 | 29.23 ± 0.86 ### |
| CSE + CBS activity, pmol of H2S mg−1 min−1 |
8.80 ± 0.22 | 30.15 ± 2.95 *** | 7.71 ± 0.11 ** | 16.48 ± 0.81 ## |
Control hearts were perfused by Langendorff preparation about 20 min. * p < 0.05, ** p < 0.01, *** p < 0.001 versus control, ## p < 0.01, ### p < 0.001 versus I/R as calculated by one-way ANOVA with post hoc Tukey HSD test.