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Preparation of 24-well plates. The numbers 1, 2, 3, and 4 are the columns in plates, and A, B, and C are the rows in the plate. Cells were fixed on the ninth day. X denotes a treatment, (-) denotes only a change in medium.
| 1 | 2 | 3 | 4 | ||
|---|---|---|---|---|---|
| Time | Ratio 1:2 | Ratio 1:4 | C+ | C− | |
| 3rd day (48 h) | A | X | X | X | - |
| B | X | X | X | - | |
| C | X | X | X | - | |
| 5th day (96 h) | A | - | - | - | - |
| B | X | X | X | - | |
| C | X | X | X | - | |
| 7th day (144 h) | A | - | - | - | - |
| B | - | - | - | - | |
| C | X | X | X | - | |
