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. 2021 Jun 24;13(7):2178. doi: 10.3390/nu13072178

Figure 3.

Figure 3

Dose- and time-dependent effects of OLE treatment on the viability of NB spheroids. (A) Cell viability determined at indicated time points, thanks to the CellTiter-Glo® 3D Cell Viability assay. Luminescence, produced only by metabolically active cells, was recorded by TECAN Infinite 200, and recorded as relative luminescence unit (RLU). Data are expressed as mean ± SD of three independent experiments. * p < 0.05, *** p < 0.001 vs. CTR). (B) Representative phase-contrast microscopy images of spheroids taken at 120 h post OLE treatment. The ImageJ processing program was used. Scale bar: 100 μm. CTR: control; OLE: olive leaf extract; uM: micromolar; h: hours.