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. 2021 Jun 24;13(7):2178. doi: 10.3390/nu13072178

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of cell proliferation, through a G0/G1 cell cycle arrest, mediated by OLE treatment. (A) The green fluorescence intensity of Carboxyfluorescein Succinimidyl Ester (CFSE) was recorded by FCM. According to the dilution of the dye, more fluorescence means less proliferation. Insets show representative FCM experiments of CFSE assay. (B) Cell cycle progression of IMR-32 and HTLA-230 determined by Nuclear Green labeling. Insets show representative cell cycle progression assay, analyzed by FCM. Data are expressed as mean ± SD of three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTR). CTR: control; OLE: olive leaf extract; uM: micromolar; MFI: mean fluorescence intensity.