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. 2021 Jul 19;10(7):909. doi: 10.3390/pathogens10070909

Table 2.

Frequency of transformation of Aliarcobacter butzleri DQ40A1 strain using homologous and heterologous PCR fragments and genomic DNA.

Donor DNA (Nature of DNA/Strain) Resistance Marker Length of the PCR Fragment Transformants/CFU
gDNA/Campylobacter coli 873 aphA-3 - NT
gyrA PCR fragment/Ab_2811 strain C254T in gyrA gene 344 bp (6.70 ± 2.72) × 10−9
gyrA PCR fragment/Ab_2811 strain C254T in gyrA gene 1410 pb (7.85 ± 1.13) × 10−8
gDNA/CR1132ΔareB::kanR aphA-3 (KanR cassette from pUC18-K2) - (2.59 ± 0.51) × 10−7
PCR fragment/of DQ40A1ΔareB::kanR aphA-3 (KanR cassette from pUC18-K2), flanked by upstream and downstream regions of about 400 bp 1638 bp (3.96 ± 3.54) × 10−7
gDNA/Ab_2811 strain C254T in gyrA gene - (6.92 ± 7.67) × 10−7
gDNA/DQ40A1ΔareB::kanR aphA-3 (KanR cassette from pUC18-K2) - (7.65 ± 2.25) × 10−6

NT—no transformants.