Table 2.
Donor DNA (Nature of DNA/Strain) | Resistance Marker | Length of the PCR Fragment | Transformants/CFU |
---|---|---|---|
gDNA/Campylobacter coli 873 | aphA-3 | - | NT |
gyrA PCR fragment/Ab_2811 strain | C254T in gyrA gene | 344 bp | (6.70 ± 2.72) × 10−9 |
gyrA PCR fragment/Ab_2811 strain | C254T in gyrA gene | 1410 pb | (7.85 ± 1.13) × 10−8 |
gDNA/CR1132ΔareB::kanR | aphA-3 (KanR cassette from pUC18-K2) | - | (2.59 ± 0.51) × 10−7 |
PCR fragment/of DQ40A1ΔareB::kanR | aphA-3 (KanR cassette from pUC18-K2), flanked by upstream and downstream regions of about 400 bp | 1638 bp | (3.96 ± 3.54) × 10−7 |
gDNA/Ab_2811 strain | C254T in gyrA gene | - | (6.92 ± 7.67) × 10−7 |
gDNA/DQ40A1ΔareB::kanR | aphA-3 (KanR cassette from pUC18-K2) | - | (7.65 ± 2.25) × 10−6 |
NT—no transformants.