Figure 2.

Combination matrix. Cells treated with hydralazine and panobinostat (A) or valproic acid (B) at the indicated concentrations for three days. Each plate contained 6 × 6 dose matrix blocks with several concentrations of both drugs. Percentage of growth inhibition was calculated for each epidrug combination using resazurin and are representative of three biological replicates (n = 3). The combination index (CI) was calculated using CompuSyn software, where <1 indicates synergism, =1 is additive effect and >1 indicates antagonism. The percentage and color codes for growth inhibition and CI scales are shown on the left. The results that CompuSyn could not convert into CI, since the effect values were not between 0 and 1, are represented by /.