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. 2021 Jul 13;14(7):670. doi: 10.3390/ph14070670

Figure 4.

Figure 4

Effect of combined hydralazine and panobinostat treatment in DNA damage of PCa cell lines by comet assay. DNA damage evaluation was determined by measuring tail moment (tail % DNA × means of head × tail distance) of at least 50 comets per condition. Representative pictures were taken with Olympus IX51 microscope at 200 × magnification (scale bar 50 μm) (left panel). Results are represented by the mean of three biological replicates (n = 3) analyzed by Kruskal–Wallis with Dunn’s test for multiple comparisons (right panel). Drug concentrations: DU145–Hydra: 50 µM, Pano: 5 nM; PC-3, RWPE-1 and WPMY-1–Hydra: 100 µM, Pano: 10 nM; LNCaP–Hydra: 100 µM, Pano: 5 nM; 22Rv1–Hydra: 75 µM, Pano: 1 nM). * p < 0.05; ** p < 0.01; **** p < 0.0001.