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. 2021 Jul 13;14(7):670. doi: 10.3390/ph14070670

Figure 5.

Figure 5

Effect of combined epidrug treatment on cell proliferation and colony formation. (A) The percentage of cell proliferation was determined by BrdU assay at day 3 of treatment with hydralazine and/or panobinostat. Experiments were performed in triplicates (n = 3), normalized to the respective vehicle and to day 0. Mean values ± SD are shown, analyzed by Kruskal–Wallis with Dunn’s test for multiple comparisons. (B) Representative images of the colony formation assay performed in prostate cell lines exposed to hydralazine and/or panobinostat for 3 days. Colony formation was followed for 7 additional days (10 days for LNCaP). Experiments were carried out in triplicates (n = 3). The effect was compared with the vehicle condition. Drug concentrations: DU145–Hydra: 50 µM, Pano: 5 nM; PC-3, RWPE-1 and WPMY-1–Hydra: 100 µM, Pano: 10 nM; LNCaP–Hydra: 100 µM, Pano: 5 nM; 22Rv1–Hydra: 75 µM, Pano: 1 nM). * p < 0.05; **** p < 0.0001.