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. 2021 Jul 13;14(7):670. doi: 10.3390/ph14070670

Figure 7.

Figure 7

Effects of hydralazine and panobinostat exposure in prostate cell lines migration potential. Cell lines were seeded in migration invasion transwell inserts upon epidrug exposure for 24 h. The percentage of invasive and migratory cells was determined by dividing the mean counted cells by the area of the microscope viewing field and then multiplying by the entire area of the insert. The results are representative of three biological replicates (n = 3). Mean values ± SD are shown (left panel). Representative images of invasive and migratory cells stained with Crystal Violet are displayed (right panel). The inserts were photographed with stereomicroscope Olympus S2X16 using a digital camera Olympus SC180 (scale bar 1 mm) at 115 × magnification. Drug concentrations: DU145–Hydra: 50 µM, Pano: 5 nM; PC-3, RWPE-1 and WPMY-1–Hydra: 100 µM, Pano: 10 nM; LNCaP–Hydra: 100 µM, Pano: 5 nM; 22Rv1–Hydra: 75 µM, Pano: 1 nM).