TABLE 3.
The time course of the bupivacaine at the dose of 300 µg, fadolmidine at the doses of 0.3, 1, 3 and 10 µg and the combination bupivacaine 300 µg and fadolmidine 0.3, 1, 3 and 10 µg on motor score (12 = normal muscle tone, 0 = muscle tone absent), rotarod performance (s, maximum measurement time was 120 s) and body temperature (°C) were measured after intrathecal injection in rats
| Dose (µg) | 0.5 h | 1 h | 2 h | 4 h | 7 h |
|---|---|---|---|---|---|
| Motor score (0–12) | |||||
| Fado a 0.3 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 |
| Fado a 1 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 |
| Fado a 3 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 |
| Fado a 10 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 | 12 ± 0 |
| Bupivac b 300 | 3 ± 0 | 10 ± 2 | 12 ± 0 | 12 ± 0 | 12 ± 0 |
| Bupivac b 300 + Fado a 0.3 | 3 ± 0 | 8 ± 2 | 12 ± 0 | 12 ± 0 | 12 ± 0 |
| Bupivac b 300 + Fado a 1 | 3 ± 0 | 4 ± 2 | 9 ± 1 | 12 ± 0 | 12 ± 0 |
| Bupivac b 300 + Fado a 3 | 3 ± 0 | 3 ± 0 | 9 ± 2 | 11 ± 1 | 12 ± 0 |
| Bupivac b 300 + Fado a 10 | 3 ± 0 | 3 ± 0 | 8 ± 2 | 11 ± 2 | 12 ± 0 |
| Rotarod performance (s) | |||||
| Fado a 0.3 | 120 ± 0 | 120 ± 0 | 120 ± 0 | 120 ± 0 | 120 ± 0 |
| Fado a 1 | 120 ± 0 | 120 ± 0 | 120 ± 0 | 120 ± 0 | 120 ± 0 |
| Fado a 3 | 118 ± 6 | 120 ± 0 | 120 ± 0 | 120 ± 0 | 120 ± 0 |
| Fado a 10 | 91 ± 44 | 94 ± 39 | 106 ± 23 | 112 ± 22 | 120 ± 0 |
| Bupivac b 300 | 0 ± 0 | 41 ± 51 | 116 ± 12 | 120 ± 0 | 120 ± 0 |
| Bupivac b 300 + Fado a 0.3 | 0 ± 0 | 1 ± 2 | 81 ± 54 | 120 ± 0 | 120 ± 0 |
| Bupivac b 300 + Fado a 1 | 0 ± 0 | 1 ± 3 | 27 ± 39 | 66 ± 48 | 116 ± 13 |
| Bupivac b 300 + Fado a 3 | 0 ± 0 | 0 ± 0 | 24 ± 40 | 73 ± 45 | 106 ± 29 |
| Bupivac b 300 + Fado a 10 | 0 ± 0 | 0 ± 0 | 14 ± 31 | 63 ± 45 | 91 ± 42 |
| Body temperature (°C) | |||||
| Saline (10 µl) | 37.8 ± 0.6 | 37.9 ± 0.3 | 38.0 ± 0.3 | 37.9 ± 0.5 | 37.6 ±0.3 |
| Fado a 0.3 | 38.0 ± 0.3 | 38.4 ± 0.4 | 38.1 ± 0.4 | 38.1 ± 0.3 | 37.7 ± 0.5 |
| Fado a 1 | 37.5 ± 0.5 | 38.0 ± 0.4 | 37.9 ± 0.4 | 37.8 ± 0.6 | 37.7 ± 0.3 |
| Fado a 3 | 37.2 ± 0.4 | 37.0 ± 0.3 | 37.7 ± 0.4 | 37.7 ± 0.6 | 37.6 ± 0.5 |
| Fado a 10 | 36.8 ± 0.5* | 36.7 ± 0.5** | 37.3 ± 0.6* | 37.7 ± 0.4 | 37.4 ± 0.3 |
| Bupivac b 300 | 36.6 ± 0.8** | 37.4 ± 0.5 | 37.9 ± 0.3 | 37.9 ± 0.4 | 37.6 ± 0.5 |
| Bupivac b 300 + Fado a 0.3 | 37.0 ± 0.6 | 37.2 ± 0.6 | 37.9 ± 0.5 | 37.6 ± 0.4 | 37.7 ± 0.3 |
| Bupivac b 300 + Fado a 1 | 36.3 ± 0.8*** | 37.2 ± 0.8 | 37.5 ± 0.4 | 37.7 ± 0.2 | 37.6 ± 0.4 |
| Bupivac b 300 + Fado a 3 | 36.0 ± 0.8*** | 36.5 ± 1.3*** | 37.3 ± 0.7* | 37.6 ± 0.5 | 37.6 ± 0.3 |
| Bupivac b 300 + Fado a 10 | 35.5 ± 0.4*** | 35.7 ± 0.6*** | 36.7 ± 0.7*** | 37.4 ± 0.7 | 37.3 ± 0.6 |
Values are presented as mean ± SD, n = 8/dose group.
*p < .05, **p < .01, ***p < .001, significantly different from corresponding saline response.
a
Fado: fadolmidine.
b
Bupivac: bupivacaine.