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. 2021 Jul 14;13(7):2409. doi: 10.3390/nu13072409

Figure 6.

Figure 6

Caffeine modulates IL-10 and STAT1 signaling in M-MΦs. Cells were pre-treated with caffeine (0.3 mM or 1 mM) for 1 h, then stimulated with LPS (100 ng/mL) for the indicated time. (A) Representative immunoblots of phosphorylated STAT1 obtained from cell lysates of M-MΦs and bar graph represents the relative protein expression determined by densitometry. (B) IL-10 secretion was determined by ELISA from cell culture supernatants. (C) The cells were pre-treated with recombinant human IL-10 (rhIL-10) (100 ng/mL) 1 h before LPS activation and IL-1β secretion was determined by ELISA from cell culture supernatants. All results are shown as means ± SEM. (* p < 0.05, ** p < 0.01, *** p < 0.001).