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. 2021 Jul 17;13(7):2449. doi: 10.3390/nu13072449

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Metabolic reprogramming in the cumulus cells of aged infertile patients after DHEA treatment. (a) Mitochondrial function was determined by oxygen consumption rate (OCR), through utilizing the Seahorse Bioscience Analyzer. (b-g) All of the OCR values at different phases of basal respiration, maximal respiration, ATP-coupled respiration, and spare capacity, proton leak and noon-mitochondrial respiration were recorded, and the values were calculated among the young, aged and aged/DHEA groups. Oligomycin (1 uM), FCCP (1 uM), antimycin (0.5 uM), rotenone (0.5 uM). * p < 0.05 and ** p < 0.01.