Table 1.

Mouse illness score.

Score Illness	0	1	2	3	4	5
Ruffled Fur	A	A	P	P	P	P
Hunched Back	A	A	A	P	P	P
Lethargy	A	A	A	P	P	P
Squinty Eyes	A	A	A	A	P	P
Inability to Reach Food/Drink	A	A	A	A	P	P
Weight Loss	A	<5%	6–10%	11–15%	16–20%	>20%

Mice were assessed every day and scored for individual symptoms, including ruffled fur, hunched back, lethargy, squinty eye, inability to reach food or drink, and weight loss. A, absent; P, present. The minimum score was 0 for a healthy mouse and 1~5 for sickness, depending on severity. Mice with scores of 4~5 were considered moribund and were humanely euthanized.