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. 2021 Jun 30;14(7):635. doi: 10.3390/ph14070635

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Anti-α-Gal and P22 IgM antibody titers in immunized and control PBS zebrafish challenged with M. marinum. Anti-α-Gal and P22 IgM antibody titers were characterized by ELISA. The o.d. at 450 nm (mean of the duplicate well values of o.d. P22 or α-Gal – o.d. PBS control) was compared between different time points (T1 to T4) by one-way ANOVA test (p < 0.05), followed by post hoc Holm multiple comparisons between T1 and T4 (* p < 0.01, ** p < 0.04, *** p < 0.05, n = 3–20/group). The time of infection challenge with M. marinum is shown with red arrows.