Table 4.

Oligonucleotide primer sequences an annealing condition.

Genes	Oligonucleotide Forward (F) and Reverse (R) Primers	Annealing Conditions	References
ccr6a	F: 5′-AGCTTCTGCGTGGCATCTAT-3′ R: 5′-CAGACGGCTGCACAAACTAA-3′	56 °C, 30 s	[24]
tlr2	F: 5′-TGAATGGGTCGAGGAGATTC-3′ R: 5′-CACAAAGTGCTCCGACAGAA-3′	56 °C, 30 s	[24]
akr2	F: 5′-ACTATGGACTTCGATCCGCT-3′ R: 5′-GCTCTGTGGTGAGTGCTGAA-3′	56 °C, 30 s	[24]
IL-1ß	F: 5′-GCATGTCCACATATGCGTCG-3′ R: 5′-GCTGGTCGTATCCGTTTGGA-3′	58 °C, 30 s	[24]
C3	F: 5′-ACGCTCTCTGGATTGAAACA-3′ R: 5′-TGCCTTCTTGCATGGCAATC-3′	56 °C, 30 s	[24]
IL-6	F: 5′-TCAACTTCTCCAGCGTGATG-3′ R: 5′-TCTTTCCCTCTTTTCCTCCTG-3′	56 °C, 30 s	[16]
tnf-α	F: 5′-AAGGAGAGTTGCCTTTACCG-3′ R: 5′-ATTGCCCTGGGTCTTATGC-3′	54 °C, 30 s	[16]
NF-kB	F: 5′-AAGAGGACCAAAATAAGCACAG-3′ R: 5′-AAGTCCAAGGTACATCGCCATGA-3′	58 °C, 30 s	[16]
hk-1	F: 5′-ACTTTGGGTGCAATCCTGAC-3′ R: 5′-AGACGACGCACTGTTTTGTG-3′f	56 °C, 30 s	[16]