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. 2021 Jul 5;13(7):2310. doi: 10.3390/nu13072310

Figure 3.

Figure 3

The effect of MFGM supplementation with exercise from a young age on age-related NMJ alterations. (A) Representative NMJ images at 6 months old (YOUNG) and 23 months old (CONT, MFGM, EX-CONT, EX-MFGM) are shown. Postsynaptic AChRs were stained with fBTX (red), and presynaptic nerve terminals were stained with antibodies against synapsin I (green). Arrows show fragmented AChRs, arrowheads denervated NMJs. Scale bar: 50 µm. The percentages of fragmented AChRs (B) and denervated NMJs (C) were determined. Values are presented as the mean ± SE (n = 4–5 in each group). ** p < 0.01 vs. YOUNG group, † p < 0.05 vs. CONT group, ‡ p < 0.05 vs. MFGM diet group by Tukey’s test. AChR, acetylcholine receptor; fBTX, fluorescently tagged α-bungarotoxin; MFGM, milk fat globule membrane; NMJ, neuromuscular junction; Syn1, synapsin I; YOUNG, young group; CONT, control diet group; MFGM, milk fat globule membrane diet group; EX-CONT, control diet with exercise group; EX-MFGM, milk fat globule membrane diet with exercise group.