Figure 3.

Effects of frankincense-based remedies on mPGES-1 activity. (a) Microsomal preparations of IL-1β-stimulated A549 cells were incubated with 2, 10, or 50 µg/mL of frankincense-based remedies or vehicle (0.1% EtOH) for 15 min at 4 °C. The reaction was started with 20 µM PGH₂. After 1 min at 4 °C, the reaction was terminated using a stop solution containing FeCl₂ and 11β-PGE₂. Formed PGE₂ was purified by SPE and analyzed using RP-HPLC. Data, expressed as percentage of control, are given as mean ± S.E.M., n=5; repeated measures ANOVA + Dunnett post hoc test with raw data; ** p < 0.01; *** p < 0.001 vs. vehicle. (b) MK-886 (10 µM) was used as positive control under the same experimental conditions as above. Paired t-test; ** p < 0.01 vs. vehicle.