Figure 3.

Level of complement component 3 (C3) in protein corona of plasmid DNA and CPP-pDNA nanoparticles. CPP-pGL3 nanoparticles or pGL3 alone were immobilized on streptavidin-superparamagnetic beads, or beads were left untreated (control) before incubation with murine serum (a,b) or murine plasma (c,d) for 5 or 60 min at room temperature. After pull-down, bound C3 subunits and its fragments were quantified by Western blot. Data represents at least three independent experiments, ±SEM, analysed by one-way ANOVA with Dunnett’s test, * p-Value < 0.05, ** p-Value < 0.005.