Table 1.
Characteristics of cell-penetrating peptides (CPPs) and nucleic acids used in nanoparticles.
| Name | Sequence | MW (kDa) | Nucleobases | Strands |
|---|---|---|---|---|
| Cell-penetrating peptides | ||||
| PF14 | Stearyl-AGYLLGKLLOOLAAAALOOLL | 2.406 | ||
| C22-PF14 | Behenyl-AGYLLGKLLOOLAAAALOOLL | 2.462 | ||
| NF55 | Stearyl-AGYLLGO * INLKALAALAKAIL | 2.378 | ||
| Nucleic acids | ||||
| SCO | CCU CUU ACC UCA GUU ACA # | 6.098 | 18 | single-stranded |
| SCO-Cy5 | Cy5-CCU CUU ACC UCA GUU ACA # | 6.760 | 18 | single-stranded |
| b-SCO | biotin-CCU CUU ACC UCA GUU ACA # | 6.485 | 18 | single-stranded |
| pDNA (pGL3) | ~3055 | ~9400 | double-stranded | |
* glycine is coupled to side chain of ornithine, instead of α-amino group. # all nucleobases have 2′OMe modification and phosphorothioate backbone.