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. 2021 Jul 17;13(7):2448. doi: 10.3390/nu13072448

Figure 2.

Figure 2

To evaluate the effect of asiatic acid (AA), (A) cell viability in HaCaT cells was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetazolium bromide assay. CON, control. (B) Effects of AA on the inflammation mechanism of tumor necrosis factor (TNF)-α- and interferon (IFN)-γ-treated HaCaT cells. According to Duncan’s multiple range test, significant values are indicated by different letters (p < 0.05), and values express the mean ± standard deviation (n = 3). Normal, control cells; Only, 20 ng/mL TNF-α- and IFN-γ-treated cells; AA-5, -10, and -20, cells pretreated with 5, 10, and 20 µg/mL AA, respectively, and then treated with 20 ng/mL TNF-α and IFN-γ.