Table 2.
Species, clinical status, and results of conventional and molecular methods in symptomatic and asymptomatic dogs and cats.
| Conventional Methods | Molecular Method | ||||||
|---|---|---|---|---|---|---|---|
| Species | Clinical Status | Sample ID | IFAT Titer | Parasite Culture | Microscopy-Histology | qPCR Blood (Leishmania/mL) |
qPCR Lymph Node (Leishmania/mL) |
| Canine | Symptomatic | 1 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
45 | 540 |
| 2 | 1:5120 | Blood + Lymph node + |
Blood + Lymph node + |
590 | 15,680 | ||
| 3 | 1:640 | Blood − Lymph node + |
Blood − Lymph node + |
30 | 970 | ||
| 4 | 1:1280 | Blood − Lymph node + |
Blood − Lymph node + |
120 | 7838 | ||
| 5 | 1:320 | Blood − Lymph node + |
Blood − Lymph node + |
75 | 660 | ||
| 6 | 1:2560 | Blood − Lymph node + |
Blood − Lymph node + |
60 | 9275 | ||
| 7 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 50 | ||
| 8 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 100 | ||
| 9 | 1:5120 | Blood + lymph node + |
Blood + Lymph node + |
645 | 24,890 | ||
| 10 | 1:160 | Blood − Lymph node + |
Blood − Lymph node + |
10 | 390 | ||
| 11 | 1:160 | Blood − Lymph node + |
Blood − Lymph node + |
20 | 460 | ||
| 12 | 1:640 | Blood − Lymph node + |
Blood − Lymph node + |
285 | 5300 | ||
| 13 | 1:320 | Blood − Lymph node + |
Blood − Lymph node + |
230 | 910 | ||
| 14 | 1:2560 | Blood − lymph node + |
Blood − Lymph node + |
185 | 19,020 | ||
| 15 | 1:2560 | Blood + lymph node + |
Blood+ Lymph node + |
580 | 17,535 | ||
| 16 | 1:320 | Blood − Lymph node − |
Blood − Lymph node + |
150 | 4230 | ||
| 17 | 1:1280 | Blood − Lymph node + |
Blood − Lymph node + |
320 | 12,545 | ||
| 18 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
35 | 850 | ||
| 19 | 1:5120 | Blood + lymph node + |
Blood + Lymph node + |
495 | 1,000,000 | ||
| 20 | 1:1280 | Blood + Lymph node + |
Blood + Lymph node + |
320 | 6710 | ||
| Asymptomatic | 21 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 10 | |
| 22 | 1:40 | Blood – Lymph node − |
Blood − Lymph node − |
Negative | 20 | ||
| 23 | 1:160 | Blood − Lymph node + |
Blood − Lymph node − |
10 | 120 | ||
| 24 | 1:40 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 15 | ||
| 25 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 20 | ||
| 26 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 15 | ||
| 27 | 1:40 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 30 | ||
| 28 | 1:40 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 65 | ||
| 29 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 80 | ||
| 30 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 35 | ||
| Feline | Symptomatic | 31 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 5 |
| 32 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 10 | ||
| 33 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 20 | ||
| 34 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 5 | ||
| 35 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 10 | ||
| 36 | 1:640 | Blood − Lymph node + |
Blood − Lymph node + |
250 | 980 | ||
| 37 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 20 | ||
| 38 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 25 | ||
| 39 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 30 | ||
| 40 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 55 | ||
| 41 | 1:320 | Blood − Lymph node − |
Blood − Lymph node + |
10 | 150 | ||
| 42 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 15 | ||
| 43 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 10 | ||
| 44 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 35 | ||
| 45 | 1:80 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 10 | ||
| 46 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 5 | ||
| 47 | 1:160 | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 50 | ||
| 48 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 5 | ||
| 49 | 1:320 | Blood − Lymph node − |
Blood − Lymph node − |
5 | 350 | ||
| 50 | Negative | Blood − Lymph node − |
Blood − Lymph node − |
Negative | 60 | ||
IFAT, indirect immunofluorescent assay; qPCR, quantitative PCR.