Figure 1.

Generation of recombinant adenoviruses expressing the FMDV 3D RNA polymerase. (A), Vero cells were mock infected or infected with the indicated recombinant adenoviruses at an MOI of 1 IFU/cell and fixed at 24 hpi. Upper panel shows phase contrast image of selected fields and lower panel shows GFP expression as analyzed by fluorescence microscopy. (B), Vero cells were mock infected or infected as indicated at an MOI of 10 IFU/cell and harvested at 24 hpi. Samples were analyzed by Western blot using antibodies against FMDV 3D protein or GAPDH as shown. A control lane containing 10 ng of purified recombinant FMDV 3D protein (r3D) was included as a reference and specificity control. The position of MWM (kDa) is indicated on the left.