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. 2021 Jul 9;10(7):869. doi: 10.3390/pathogens10070869

Figure 2.

Figure 2

Western blot analysis of SARS-CoV-2 spike protein with V5 tag at its C-terminus using samples from Vero cells transiently transfected with either vector or with the plasmid-expressing full-length spike protein, which will be cleaved into S1 and S2 in the cells [28], as indicated. (A) Antibody against V5 tag could detect full-length (FL) and cleaved S2 of the spike protein with the V5 tag at its C-terminus [18]. (B) Anti-S (a.a. 440–460) antibodies (14745 and 14746) could react with full-length (FL) and cleaved S1 of the spike protein. (C) Anti-S (a.a. 494–506) (14901 and 14902) could react with full-length (FL) and cleaved S1 of the spike protein. A nonspecific band between FL and S1, marked as *, could react with Anti-S (a.a. 494–506).