Figure 3.

Internalization of the trastuzumab-DNA mimic conjugate in HER2 cells expressing HER2 at 4 °C and 37 °C as evaluated by immunofluorescence. Exponentially growing cells were incubated with 15 µg/mL of trastuzumab (TZ) or the trastuzumab-DNA mimic conjugate (ADC) for 2 h at 4 °C or 37 °C and were fixed. Localization of the antibodies was revealed by immunofluorescence using an FITC labeled antibody as described in the Materials and Methods section. Untreated cells were used as a negative control.