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. 2021 Jul 15;13(7):1082. doi: 10.3390/pharmaceutics13071082

Figure 4.

Figure 4

Effect of schisandrol A and 17β-estradiol (E2) on the absence or presence of ICI 182,780 (ICI) in the protein expression of phospho-extracellular signal-regulated kinase (p-ERK), ERK, phospho-phosphatidylinositol 3-kinase (p-PI3K), PI3K, p-Akt, Akt, phospho-estrogen receptor α (p-ERα), and ERα in MCF-7 cells: (A) protein expression levels of p-PI3K, PI3K, p-Akt, Akt, p-ERα, and Erα and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in untreated cells and 50 μM and 100 μM schisandrol A-treated MCF-7 cells (24 h). (B–E) Bar graph representing the densitometric quantification of Western blot bands. * Significant difference between cells treated with schisandrol A or E2 and the untreated cells. # Significant reduction in co-treatment with ICI compared to treatment with compounds alone (n = 3 independent experiments, p < 0.05, Kruskal–Wallis nonparametric test). Data are represented as mean ± SEM.