Figure 8.

Relative normalized expression of genes that regulate biosynthesis of ABA (A,B), GA (C,D), and ethylene (E,F) in the floral buds of peach ‘Redhaven’. Expression of each gene was normalized to that of β-Actin and expressed relative to the control sample (20 CH). Each point represents the mean ± SE of three biological replicates, each with three technical replicates. Values marked with asterisks * and ** are significantly different at p < 0.05 and 0.01, respectively. NCED, 9-cis-epoxycarotenoid dioxygenase; GA20-OX, GA 20-oxidase; ACS, 1-aminocyclopropane-1-carboxylic acid (ACC)-synthase; and ACO, ACC-oxidase. CH, chilling hour; GDH, growing degree hour. Refer to Figure 7 legend for the sampling dates that correspond to the CH and GDH accumulation.