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. 2021 Jul 16;13(7):1091. doi: 10.3390/pharmaceutics13071091

Figure 3.

Figure 3

Apoptosis induction by 1A-116 is higher at 10 HPS in LN229 cells. Annexin V staining was used to measure early apoptosis in LN229 cultures 6 h after the addition of vehicle, 20 μM, or 50 μM of 1A-116. Fluorescence intensity was measured in a Cytation 5 system and DAPI staining was used to relativize the number of cells in each well. (A) Relative early apoptosis values were obtained for three independent experiments (N = 3) and were compared between treatments at 10 HPS and 23 HPS. Significantly larger values were observed when the treatment was applied at 10 HPS (two-way ANOVA, for factor HPS p < 0.01, factor Concentration p < 0.001, interaction p < 0.05. Multiple comparisons Tukey´s tests: 0 μM 23 HPS vs. 20 μM 10 HPS: ** p < 0.01, 0 μM 10 HPS vs. 20 μM 10 HPS: ** p < 0.01, 0 μM 23 HPS vs. 50 μM 23 HPS: ** p< 0.01, 0 μM 10 HPS vs. 50 μM 10 HPS: **** p < 0.0001, 0 μM 23 HPS vs. 50 μM 10 HPS: **** p < 0.0001, 20 μM 23 HPS vs. 20 μM 10 HPS: ** p < 0.01, 20 μM 23 HPS vs. 50 μM 23 HPS: ** p < 0.01).Column values represent mean ± SEM. (B) Representative images that were obtained to qualitatively confirm the staining results in LN229 cells at 10 HPS and 23 HPS, 6 h after treatment with 1A-116. White scale bars represent 20 μm.