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. 2021 Jul 6;13(7):1028. doi: 10.3390/pharmaceutics13071028

Figure 4.

Figure 4

Effects of Gancaonin N on protein levels of pro-inflammatory cytokines (A–C) and COX-2 (D) in LPS-induced A549 cells. Gancaonin N (5–40 μM) was pretreated 2 h prior to LPS-induced inflammation in A549 cells, incubated for 24 h with LPS (5 μg/mL). The protein level of inflammatory biomarkers was investigated by immunoblotting assay. Ratio of each protein was determined by ImageJ. Values are represented as means ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 are significantly different from non-treated group; * p < 0.05, ** p < 0.01, *** p < 0.001 are significantly different from only LPS-treated group.