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. 2021 Jul 1;13(7):1003. doi: 10.3390/pharmaceutics13071003

Figure 2.

Figure 2

Figure 2

Gene transfection effects with various ternary complexes. Cells (5 × 104 cells /well) were incubated with ternary complexes containing pDNA (1 μg/well) for 4 h. Luciferase activity was analyzed 2 days after the transfection. The influence of ratio of cationic polymer in ternary complexes on the transfection in (a) Neuro2a cells and (b) HeLa cells. (c) Gene transfection effects with LF2000. Neuro 2a cells or HeLa cells were incubated with LF2000 complexes containing pDNA (1 μg/well) for 4 h. Luciferase activity was analyzed 2 days after the transfection. The bars show the mean and S.D. (n = 3). * indicates p < 0.05 using an unpaired t-test. (d) The influence of N/P ratio of ternary complexes (PEG-PEI:RVG-R9 = 4:1) on the transfection in Neuro2a cells. The bars show the mean and S.D. (n = 3). * indicates p < 0.05 using a one-way ANOVA with Tukey’s post-hoc test. n.s. indicates no significant difference.