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. 2021 Jul 1;13(7):1003. doi: 10.3390/pharmaceutics13071003

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Specific interaction with ternary complexes and Neuro2a cells. (a) Neuro2a cells and (b) HeLa cells were incubated with ternary complexes containing YOYO-1 (green) labeled pDNA (0.4 μg/well) for 2 h. Cells were fixed, stained with DAPI (blue), and evaluated by fluorescence microscopy. Scale bars represent 10 μm. (c) The influence of preincubation with RVG peptide for 1 h on the transfection by ternary complexes (pDNA 1 μg/well, N/P 10, PEG-PEI:RVG-R9 = 8:2). The bars show the mean and S.D. (n = 3). * indicates p < 0.05 using a one-way ANOVA with Tukey’s post-hoc test.

Specific interaction with ternary complexes and Neuro2a cells. (a) Neuro2a cells and (b) HeLa cells were incubated with ternary complexes containing YOYO-1 (green) labeled pDNA (0.4 μg/well) for 2 h. Cells were fixed, stained with DAPI (blue), and evaluated by fluorescence microscopy. Scale bars represent 10 μm. (c) The influence of preincubation with RVG peptide for 1 h on the transfection by ternary complexes (pDNA 1 μg/well, N/P 10, PEG-PEI:RVG-R9 = 8:2). The bars show the mean and S.D. (n = 3). * indicates p < 0.05 using a one-way ANOVA with Tukey’s post-hoc test.