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. 2021 Jun 26;13(7):968. doi: 10.3390/pharmaceutics13070968

Figure 10.

Figure 10

The effect of PtPz4, PtPz6, cisPt, and anti-MUC1 on caspase-9 mRNA (A), pro- and cleaved caspase-9 (B), caspase-3 mRNA (C), and pro- and cleaved caspase-3 (D) expressions. The cells were incubated for 24 h with PtPz4 (10 μM), PtPz6 (10 μM), cisPt (10 μM), anti-MUC1 (5 μg/mL), PtPz4 + anti-MUC1 (10 μM + 5 μg/mL), PtPz6 + anti-MUC1 (10 μM + 5 μg/mL), and cisPt + anti-MUC1 (10 μM + 5 μg/mL). mRNAs were assessed by RT-PCR. The result is presented as a relative fold change in mRNA gene expression in comparison of gene in control where expression was set at 1 ± SD are the mean of triplicate cultures. * p < 0.05; ** p < 0.01; *** p < 0.001. Pro- and cleaved caspase expressions in cell lysates were assessed by Western blotting. The intensities of the bands were quantified by densitometric analysis. Data represent the mean ± SD of the relative expression levels (from three assays) normalized to β-actin. * p < 0.05; ** p < 0.01; *** p < 0.001.