The estimated mean recovery of cryopreserved buds of blackcurrant cvs. Marski, Mikael, and Vilma according to (a) the pre-treatment of bud sections (desiccated or not) and the bud length, and (b) the bud type and rehydration time of bud sections. The effects of cultivar, pre-treatment, thawing method, rehydration, type of bud, size of bud, and all their 2-way and 3-way interactions were analysed using generalised linear mixed models. The length of the bars indicates the sizes of 95% confidence intervals. Letters a and b indicate significant differences (p < 0.05) in estimated recovery rate in vitro between treatments.