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Immunofluorescence study for the effects of ZO essential oil at the dilution of 1:25,000 on (A) pERK1/2 and (B) pAkt activation. HeLa cells were stained with Hoechst 33342 for nuclear staining (blue). Micrographs were photographed by a fluorescent microscope (100× magnification). (C) Western blot analysis detecting the phosphorylation (activation) status of ERK1/2 and Akt kinases and their total protein expression.
