Figure 10.

Sperm viability classification in the control group (A,B), the red NAE 0.44 mg/mL of (C) and the white NAE 0.44 mg/mL (D) stained with DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride) and PI (Propidium iodide). The sperm was stained blue with DAPI and stained red with PI (DAPI+PI+) was considered dead, but sperm that was stained blue with DAPI and remained unstained with PI (DAPI+PI-) was viable spermatozoa. The investigated sperm was photographed at a magnification of 400× under ImageXpress Micro 4 High-Content Imaging System.