Table 1.
Physicochemical characterization and in silico analysis of synthetic peptide RcAlb-PepIII.
| Properties | RcAlb-PepIII | Kalata B2 |
|---|---|---|
| Physicochemical | ||
| Sequence | AKLIPTIA | GLPVCGETCFGGTCNTPGCSCTWPICTRD |
| 1 Calculated molecular mass (Da) | 826.05 | 2979.81 |
| 2 Isoeletric point (pI) | 8.80 | 4.71 |
| 1 Hydrophobicity | 0.864 | 0.634 |
| 1 Net charge | +1 | −1 |
| 4 Ramachandran Plot (%) | 98 | 34 |
| 5 Tm | 0.56 | - |
| 5 sOPEP | −5.73 | - |
| Biological Properties | ||
| 6 CPP | No | Yes |
| 7 Allergic potential | No | Yes |
| Hemolytic potential | No | Yes |
| Toxic potential | No | Yes |
| 9 Cleavage sites | ||
| Trypsin (high pH) | 1 | 1 |
| Pepsin (pH 1.3) | 1 | 3 |
| Pepsin (pH > 2) | 1 | 4 |
| 10 Half-life time | 2.21 | 0.961 |
| 10 Stability | High | Normal |
1 Calculated using the Antimicrobial Peptide Database. 2 Calculated using the ExPASy ProtParam toll. 4 Obtained using Rampage. 5 Tm and sOPEP scores were calculated by the PEP-FOLD server. 6 Cell-Penetrating Peptide (CPP) capacity was calculated using CellPPD tool. 7 Allergic potential was calculated using the Antigenic prediction tool. 8 Antimicrobial potential was calculated using the iAMPpred tool. 9 Cleavage sites were calculated using the Peptide Cutter tool. 10 Half-life time in intestinal environment and stability were calculated using the HLP tool.