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. 2021 Jul 6;13(7):1310. doi: 10.3390/v13071310

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Schematic workflow of the modified dsRNA-seq method. (1) Preparation of liver tissues and homogenization. (2) Extraction of nucleotide contained in dsRNA from clinical samples using phenol/chloroform extraction. (3) Purification of dsRNA using cellulose column chromatography. (4) Enzymatic removal of host-derived DNA and ssRNA from the purified dsRNA. (5) Fragmentation by Covaris S220. (6) Thermal denaturing. (7) Synthesis of cDNA by annealing random primers. cDNAs obtained from this method were analyzed by Hiseq3000. Details of this method are described in the Materials and Methods section.