Figure 2.

Production and biophysical characterization of PHA beads. (A) Schematic representation of hybrid genes encoding fusion proteins for the production of PHA, Ag-PHA and PHA-PopB beads in P. aeruginosa PAO1ΔCΔ8ΔF strain. MW (Molecular weight). phaC1 is the PHA synthase gene. (B) Table showing PHA beads composition and yield data. (C) TEM images for accumulation and size analysis of PHA beads in whole-cell and isolated PHA beads fractions. (D) Size of PHA beads before and after formulation with Alhydrogel adjuvant. Polydispersity indices are shown above the beads. (E) Zeta potential of PHA beads before and after formulation with Alhydrogel adjuvant. The particle size and zeta-potential of each PHA beads were measured three times by Zetasizer Nano ZS. Each data point of measurement represents the mean ± SEM. The values are shown in Table S3.