Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 15;13(7):1377. doi: 10.3390/v13071377

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Pulldown assays of full-length Ms6 LysB and Ms6LysBPGBD-EGFP and EGFP with purified peptidoglycan from: (a) M. smegmatis, E. coli, P. aeruginosa and B. subtilis. Pellet and supernatant fractions were analyzed by SDS-PAGE followed by Comassie-blue staining. Bovine serum albumin (BSA) was used as a control and was only present in the supernatant fractions. (b) As a negative control, Ms6 LysB was incubated without peptidoglycan. (c) Quantification of pulldown assays. Quantification was performed with 3 independent gels, including the represented in Panel A. Ms6 LysB (dark grey), Ms6 LysBPGBD-EGFP (ligth grey). For each bar, the mean ± SD of three independent assays is indicated. (d) Pulldown assays of full length Ms6 LysB with purified peptidoglycan from S. pneumoniae and S. aureus. Again, BSA was used as a control and is only present in the supernatant fractions.