Table 3.
Viral titers (10x TCID50/100 µL organ homogenate).
| Organs | Sheep | |||||
|---|---|---|---|---|---|---|
| N2 | M1 | M2 | M3 | M4 | P4 | |
| Lung | - | - | 1.0 | 2.5 | - | 2.5 |
| Liver | - | - | 1.0 | 1.5 | 1.5 | 2.75 |
| Lnn. hepatici | - | 2.0 | - | 2.0 | - | - |
| Spleen | - | 2.75 | 2.5 | 3.0 | 0.75 | 3.0 |
| Jejunum | - | - | - | - | - | 1.5 |
| Ileum | - | 1.75 | - | - | - | - |
| Colon | 0.75 | 1.0 | - | - | - | 1.0 |
| Rectum | 1.0 | 2.75 | - | - | 1.0 | - |
| Lnn. mesenteriales | - | 0.75 | - | 0.75 | - | - |
| Kidney | - | - | - | - | 0.75 | - |
| Conchae | 1.75 | 2.25 | 3.5 | 4.25 | 1.25 | 1.25 |
| Conjunctiva | 1.75 | 1.0 | 2.0 | 3.0 | - | 2.5 |
| Injection site | 0.75 | 1.0 | - | 1.75 | 1.0 | - |
| Ln. cervicalis superf. | - | - | - | 1.75 | - | - |
| Lnn. iliaci med | - | - | - | 1.5 | 0.75 | - |
| Celiac ganglion | - | 1.0 | - | 1.0 | - | - |
All organ samples collected during the necropsies of the calves were also analyzed by RT-qPCR. Low equivalents of viral genome copies were detected in lymphatic tissues (R5 Ln. cervicalis cran.: 6 copies/mg; Q5 Lnn. retropharyngeales: 2.5 copies/mg and Q6 Lnn. iliaci mediales: 47.2 copies/mg).