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. 2021 Jul 19;9(7):800. doi: 10.3390/vaccines9070800

Figure 4.

Figure 4

Protective efficacy of B/Bris PB1att 4M vaccine against homologous challenge. Vaccinated (B/Bris PB1att 4M) or unvaccinated (mock-challenged) DBA/2J mice were challenged with the homologous lethal strain B/Bris PB2-F406Y 21 days post-boost. A non-vaccinated, non-challenged control group was included (mock-mock). (A) Percentage body weight change and (B) survival rate were evaluated daily for 12 days post-challenge. (C) The serum antibody response against the homologous (B/Bris wt) virus was measured by HI assay at 14 days post-challenge (dpc). Shaded area indicates cutoff for HI titers considered to be predictive of protection. Weight loss data were compared using two-way ANOVA corrected for multiple comparisons using Holm–Sidak’s test. Survival curves were compared using log-rank Mantel–Cox curve comparison. Significant differences, compared with the mock-challenged group, are indicated. * p < 0.05, *** p < 0.001. Plotted data represent means ± standard errors.