Figure 4.

RBP confirmation screen summary. (A) transformants with known plasmid constructs were lifted onto nitrocellulose membranes and subjected to the RBP identification assay. Levels of host cell binding were phenotypically assessed compared to the controls (membranes containing empty pET30a or fragments identified in initial screen). (B) results following staining of membranes containing full length or truncated versions of Gp4 as well as positive and negative controls. Binding was graded as follows: (+++) specific binding in all or majority of areas containing protein, (++) binding in some areas containing protein, and (+) mostly nonspecific bacterial growth throughout the membrane.