Table 1.
Strains and plasmids.
| Strain/Plasmid | Relevant Characteristics a | Source or Reference |
|---|---|---|
| Strain | ||
| E. coli | ||
| BL21 (DE3) | F− ompT hsdSB (rB− mB−) gal dcm λ (DE3) | Novagen |
| DH5α | recA1 endA1 | [53] |
| Lemo21 (DE3) |
fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS/ pLemo(Cmr) pLemo = pACYC184-PrhaBAD-lysY |
NEB |
| S17-1 | recA endA thi hsdR RP4-2 Tc::Mu::Tn7 Tpr Smr | [54] |
| TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (Smr) endA1 nupG | Gift from Earl Petzold |
| Agrobacterium sp. H13-3 | ||
| RU12/001 | Smr; spontaneous streptomycin-resistant wild-type strain | [55] |
| RU12/006 | Smr; ΔflaA, ΔflaB, ΔflaD | [55] |
| RU12/012 | Smr; ΔmotA | [42] |
| RU12/015 | Smr; ΔAGROH133_08824 | This study |
| RU12/016 | Smr; ΔAGROH133_07337 | This study |
| RU12/017 | Smr; ΔAGROH133_13050 | This study |
| RU12/023 | Smr; ΔfliK | This study |
| Plasmid | ||
| pET30a | Kmr, expression vector (N- or C- terminal 6x histidine tag) | Gift from Christine Szymanski |
| pBS1218 | Kmr, 7-7-1_0004 fragment (741 bp) cloned into pET30a restriction sites NcoI/XhoI with N-terminal 6x histidine tag | This study |
| pBS1219 | Kmr, 7-7-1_0004 fragment (741 bp) cloned into pET30a restriction sites BamHI/SacI with C-terminal 6x histidine tag | This study |
| pBS1220 | Kmr, full length 7-7-1_000102 (1758 bp) cloned into pET30a restriction sites NcoI/BamHI with N-terminal 6x histidine tag | This study |
| pBS1221 | Kmr, full length 7-7-1_000102 (1761 bp) cloned into pET30a restriction sites NcoI/XhoI with C-terminal 6x histidine tag | This study |
| pBS1222 | Kmr, full length 7-7-1_0004 (1362 bp) cloned into pET30a restriction sites BamHI/SacI with N-terminal 6x histidine tag | This study |
| pBS1223 | Kmr, full length 7-7-1_00044 (348 bp) cloned into pET30a restriction sites BamHI/SacI with N-terminal 6x histidine tag | This study |
| pBS1224 | Kmr, full length 7-7-1_00044 (348 bp) cloned into pET30a restriction sites SalI/XhoI with C-terminal 6x histidine tag | This study |
| pBS1225 | Kmr, 7-7-1_00044 fragment (189 bp) cloned into pET30a restriction sites BamHI/SacI with N-terminal 6x histidine tag | This study |
| pBS1226 | Kmr, 7-7-1_00044 fragment (189 bp) cloned into pET30a restriction sites SalI/XhoI with C-terminal 6x histidine tag | This study |
| pBS1227 | Kmr, 7-7-1_000102 fragment (222 bp) cloned into pET30a restriction sites NcoI/XhoI with C-terminal 6x histidine tag | This study |
| pBS1228 | Kmr, full length 7-7-1_0004 cloned into pET30a restriction sites NcoI/XhoI with C-terminal 6x histidine tag | This study |
| pK18mobsacB | Kmr; lacZ mob sacB | [56] |
a Cmr, chloramphenicol resistance; Smr, streptomycin resistant; Kmr, kanamycin resistant; bp, base pairs.