. 2021 Jun 29;13(7):1267. doi: 10.3390/v13071267

Table 4.

Confirmatory RBP screen binding analysis.

Gene	Plasmids	Protein Expressed	Binding ^c
7-7-1_0004	pBS1218	6xHis-Gp4_1-247	+++
	pBS1219	Gp4_1-247-6xHis	++
	pBS1222	6xHis-Gp4	+
	pBS1228	Gp4-6xHis	+
	FG19-1	Gp4_1-246-6xHis ^a	++
	pET30a	---	+
7-7-1_000102	pBS1220	6xHis-Gp102	+
	pBS1221	Gp102-6xHis	++
	pBS1227	Gp102_363-436-6xHis	+
	FG9	6xHis-Gp102_363-436	+++
	pET30a	---	+
7-7-1_00044	pBS1223	6xHis-Gp44	+
	pBS1224	Gp44-6xHis	+++
	pBS1225	6xHis-Gp44_53-115	++
	pBS1226	Gp44_53-115-6xHis	+
	FG5	6xHis-Gp44_53-114 ^b	+++
	pET30a	----	+

^a Fragment ended with two nucleotides that were not in frame; therefore, pBS1218 and pBS1219 include an extra nucleotide to generate a fragment with an additional native codon. ^b Fragment ended with two nucleotides that were not in frame; therefore, pBS1225 and pBS1226 include an extra nucleotide to generate a fragment with an additional native codon. ^c (+++) denotes specific binding in areas containing protein, (++) indicates mostly specific binding with some background growth, and (+) represents nonspecific bacterial growth throughout the membrane (background).