Figure 4.
SgP-regulated luciferase expression to determine CHIKV-neutralizing antibodies. (A): CHIKV infection of TR-RNA-luc-transfected HEK 293T cells in the presence of three human plasma samples from convalescent CHIKV-infected patients at a dilution ranging from 10−2 to 10−7. Infection was determined as luciferase activity in counts/s and is presented as % of untreated control on a log scale. The values represent mean values ± SD of triplicate measurements. (B): CHIKV infection in the presence of human naïve serum. (C): Area under the curve (AUC) values were calculated using the GraphPad Prism 7.04 software. The reciprocal AUC values of neutralization assays with plasma from CHIKV- or RRV-infected patients and MAYV-infected mouse sera, or naïve serum samples, are depicted to illustrate CHIKV-specific neutralization activity.